Bazična istraživanja

Background: The biological examination of pulp injury, repair events and response of dental pulp stem cells to dental
restorative materials is important to accomplish restorative treatment, especially to commonly used dental materials in
paediatric dentistry, such as glass ionomer cement (GIC) and calcium hydroxide (Ca(OH)2) lining cement.
Methods: Healthy patients aged between 9 to 11 years with carious primary molars without pulp exposure were selected
and divided into two groups: Group 1 (teeth restored with GIC) and Group 2 (teeth lined using Ca(OH)2 and restored with
GIC). The proliferative activity of stem cells of teeth between these two groups was compared using colourimetric
cell proliferation reagent, alamarBlue. Immunocytochemistry and flow cytometry confirmation were performed using
mesenchymal stem cell markers, CD105 and CD166.
Results: The proliferative activity using alamarBlueTM assay showed that cells derived from the remaining dental pulp of
exfoliated deciduous teeth were positive for CD105 and CD166 and exhibited no difference between the two groups.
Conclusions: It can be concluded that the use of Ca(OH)2 or GIC as a lining material in indirect pulp capping procedures
has the same effect on cells derived from the remaining dental pulp of exfoliated deciduous teeth which have responded
favourably to the restorative treatments.